| PDGF-BB

Platelet-derived growth factor (PDGF) is one among numerous growth factors that regulate cell growth and division. To activate its biological functions, PDGF monomeric proteins (PDGF-A, PDGF-B, PDGF-C, and PDGF-D) must either form homodimers or heterodimers (PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, and PDGF-DD) and then bind with PDGF receptors (PDGFR-α and PDGFR-β) . PDGF-BB is the best characterized member of the PDGF family.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.26	1.45	0.85	0.55	0.36	0.27	0.19	0.07
Corrected OD	2.19	1.38	0.78	0.48	0.29	0.2	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PDGF-BB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	95-108	104

 

| Linearity

Samples were spiked with high concentrations of Human PDGF-BB and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-96	88-96	90-110
	Average (%)	92	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108